【プレスリリース】わずか60秒で巨大DNAのサイズ分析を実現

更新日：8月4日

・ナノスリット流路による巨大DNAのサイズ分析法を開発し、既存技術よりも高分離能・短時間でサイズ分析を実現。

・DNAの緩和時間（伸長状態からコイル状へ戻るまでの時間）を計測することで、サイズを正確に判別。

・デバイスは簡便なフォトリソグラフィで製造可能、わずか200分子で解析可能なため、量産・実用化に向けた展開が期待。

・新手法により、高精度かつ網羅的な疫学解析が可能、薬剤耐性菌対策への貢献が期待｡

雑誌名：Lab on a Chip

論文タイトル：Size analysis of large DNA molecules by relaxation time measurement using a nanoslit channel

著者：Hongdong Yi, Shintaro Itoh, Kenji Fukuzawa, Hedong Zhang, Naoki Azuma

DOI: 10.1039/D4LC00998C

A method for analyzing the size of giant DNA using nano-slit flow channels has been developed, enabling higher separation efficiency than existing technologies and size analysis in a short time.
The size can be accurately determined by measuring the relaxation time (the time it takes for the DNA to return from an extended state to a coiled state).
The device can be manufactured using simple photolithography and can analyze as few as 200 molecules, so it is expected to be developed for mass production and practical application.
The new method enables high-precision and comprehensive epidemiological analysis, contributing to strategies against drug-resistant bacteria.

Journal: Lab on a Chip

Title: Size analysis of large DNA molecules by relaxation time measurement using a nanoslit channel

Authors: Hongdong Yi, Shintaro Itoh, Kenji Fukuzawa, Hedong Zhang, Naoki Azuma

DOI: 10.1039/D4LC00998C